粘质沙雷氏菌(Serratia marcescens)发酵培养基优化的研究   总被引：6，自引：0，他引：6  

郝林华  陈靠山  牛德庆  张玉凤 《海洋科学进展》2006,24(1):66-73

采用单因素试验和正交试验,对从山东东营海岸湿地盐碱滩地土壤中筛选出的一株海洋菌种———粘质沙雷氏菌(Serratia marcescens)的发酵培养基进行优化,并进行100 L发酵罐中试放大试验的研究。确定粘质沙雷氏菌的最佳培养基配方为葡萄糖10 g/L,硫酸铵5 g/L,麸皮50 g/L,柠檬酸三钠1.0 g/L,K2HPO4.3H2O 0.3 g/L,FeSO4.7H2O 0.05 g/L,MgSO4.7H2O 0.5 g/L,pH 7.2~7.7。发酵最适温度为30℃。通过测定粘质沙雷氏菌在发酵罐中培养的生长曲线,确定发酵时间以28~30 h为宜,发酵结束后发酵液中的活菌数约为50×108个/mL。将所筛选到的粘质沙雷氏菌应用于农作物的病害防治,效果非常显著,表明是一株高活性的生物防治拮抗菌。此研究结果为高效率、低成本和工业化生产具有生物防治作用的海洋菌种制剂提供了科学依据,也为海岸湿地盐碱土的可持续开发利用提供了技术支撑。  相似文献   

几种海水贝类甲醛浸渍标本DNA的提取及rRNA基因ITS序列的扩增   总被引：8，自引：0，他引：8  

孙博  张素萍  王海艳  刘晓  张国范 《海洋通报》2004,23(1):40-44

从中国科学院海洋生物标本馆收藏的8种甲醛浸渍的海水贝类标本中提取DNA并对其rRNA编码基因的ITS序列进行特异性扩增，在牡蛎Crassostrea sp．、长肋日月贝Amussium pleurondectes (Linnaeus)、扇贝Volachlamys ringaporinug(Sowerby)与华贵栉孔扇贝Chlamys nobilis(Reeve)等4个物种的标本中成功获得扩增产物。利用图片分析软什得出牡蛎标本的ITS-1和ITS-2扩增片段分别为408bp和524bp，长肋日月贝标本分别521bp和537bp，而扇贝Volachlamys ringaporinug(Sowerby)与华贵栉孔扇贝标本只获得ITS-1扩增片段，长度均为545bp。  相似文献   

脂质体悬浮液结晶对其冻干品质影响的研究     

刘占杰  华泽钊  陶乐仁  李保国  陈建明  高申 《中国海洋大学学报(自然科学版)》2001,31(4):612-618

利用低温显微镜系统对含有不同浓度海藻糖的脂质体悬浮液 ,在不同的降温速率下的结晶现象进行显微研究 ,得出不同条件下的冰晶生长图像 ;并研究脂质体在不同的降温速率下的冻干过程 ,比较了不同降温速率下的冻干脂质体的外观和冻干脂质体复水后的囊泡粒径变化。结果表明 ,对于液体药品快速冻结后得到的冻干品质量优于慢速冻结。  相似文献   

一类全局最优化问题的动态隧道方法     

李博  曹圣山 《中国海洋大学学报(自然科学版)》2003,33(1):160-164

研究了连续变量函数的全局最优化问题 ,给出了动态隧道方法。该动态隧道方法由局部搜索和动态隧道 2个阶段构成。在局部搜索阶段用了动态系统方法。对全局最优化问题的实例进行了数值实验 ,数值结果表明了该方法的稳健性和有效性。  相似文献   

极化SAR目标相对最优极化研究     

余海坤  张永红  汪云甲 《海洋测绘》2006,26(5):13-15,19

目标相对最优极化就是选取一种极化状态,使得研究目标和背景地物的接收功率对比达到最大。通过讨论了目标相对最优极化问题,提出了改进的模型和解算方法,利用实际数据对其模型和方法进行了实验,验证了其有效性和正确性。  相似文献   

A community-wide intercomparison exercise for the determination of dissolved iron in seawater     

Andrew R. Bowie  Eric P. Achterberg  Peter L. Croot  Hein J.W. de Baar  Patrick Laan  James W. Moffett  Simon Ussher  Paul J. Worsfold 《Marine Chemistry》2006

The first large-scale international intercomparison of analytical methods for the determination of dissolved iron in seawater was carried out between October 2000 and December 2002. The exercise was conducted as a rigorously “blind” comparison of 7 analytical techniques by 24 international laboratories. The comparison was based on a large volume (700 L), filtered surface seawater sample collected from the South Atlantic Ocean (the “IRONAGES” sample), which was acidified, mixed and bottled at sea. Two 1-L sample bottles were sent to each participant. Integrity and blindness were achieved by having the experiment designed and carried out by a small team, and overseen by an independent data manager. Storage, homogeneity and time-series stability experiments conducted over 2.5 years showed that inter-bottle variability of the IRONAGES sample was good (< 7%), although there was a decrease in iron concentration in the bottles over time (0.8–0.5 nM) before a stable value was observed. This raises questions over the suitability of sample acidification and storage.  相似文献   

Fuzzy Optimum Model of Semi-Structural Decision for Lectotype Optimization of Offshore Platforms   总被引：8，自引：0，他引：8  

CHEN  Shouyu 《中国海洋工程》2001,(4):453-466

In the process of concept design of offshore platforms, it is necessary to select the best from feasible alternatives through comparison and filter. The criterion set, used to evaluate and select the satisfying alternative, consists of many qualitative and quantitative factors. Therefore, the selection is a problem of multicriteria and semi- structural decision- making. Different from traditional methods in semi- structural decision- making, a new framework and methodology is presented in this paper for evaluation of offshore platform alternatives. First, the criterion set is established for the evaluation of alternatives. Next, the approach is studied to construct the relative membership degree matrix, in which both qualitative and quantitative factors are consistent with the uniform calculating standard. And then a new weight-assessing method is developed for calculation of the weights based on the relative membership degree matrix. Finally, a multi-hierarchy fuzzy optimum model is adopted to select t  相似文献   

Kohonen网络与BP网络的集成应用研究     

丁香乾  曹均阔  贺英 《中国海洋大学学报(自然科学版)》2003,33(4):615-620

本文介绍了 Kohonen神经网络对输入数据进行聚类方法在卷烟配方中的应用 ,提出了从核心样本动态搜索 BP网络训练样本的新探索 ,摒弃了过去 BP算法中训练样本固定不变 ,互不相交的方法 ,实现了 BP网络和 Kohonen网络动态无缝集成。  相似文献   

载人潜水器圆柱形耐压壳体的优化设计研究     

李学斌 《海洋技术学报》2007,26(3):5-8,12

采用分枝定界法和序列二次规划方法,对载人潜水器圆柱形耐压壳体的重量最小化进行了研究.设计变量是壳板的厚度、肋骨的型号、间距和数量,讨论了下潜深度、材料几何参数对重量以及其它特征量的影响.算例计算表明,下潜深度越大,屈服极限越高的材料重量减轻越明显.对于大深度而言,选用高屈服极限材料,可以使得材料能够充分利用.文中还对结构重量占排水量比例随深度变化的情况进行了研究.  相似文献   

Optimization Mathematical Model of Pile Forces for Offshore Piled Breasting Dolphins     

周锡礽  王乐芹王晖 朱福明 《中国海洋工程》2004,18(4):567-575

An optimization mathematical model of the pile forces for piled breasting dolphins in the open sea under various loading conditions is presented. The optimum layout with the well distributed pile forces and the least number of piles is achieved by the multiplier penalty function method. Several engineering cases have been calculated and compared with the result of the conventional design method. It is shown that the number of piles can be reduced at least by 10%—20% and the piles‘ bearing state is improved greatly.  相似文献